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Table 1 Set-up of microarrays.

From: Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Chip No.

Sample labelled with Cy3

Sample labelled with Cy5

Experiment

1

P. pastoris shake flask, pH 5.0

P. pastoris shake flask, pH 5.0

yellow

2

S. cerevisiae shake flask, pH 5.0

S. cerevisiae shake flask, pH 5.0

yellow

3

P. pastoris shake flask, pH 5.0

P. pastoris shake flask, pH 3.5

pH shift

4

S. cerevisiae shake flask, pH 3.5

S. cerevisiae shake flask, pH 5.0

pH shift

5

P. pastoris fed batch, glycerol, pH 5.0

P. pastoris fed batch, methanol, pH 5.0

Shift glycerol to methanol at pH 5

6

P. pastoris fed batch, methanol, pH 5.0

P. pastoris fed batch, glycerol, pH 5.0

Shift glycerol to methanol at pH 5

7

P. pastoris fed batch, glycerol, pH 3.0

P. pastoris fed batch, methanol, pH 3.0

Shift glycerol to methanol at pH 3

8

P. pastoris fed batch, methanol, pH 3.0

P. pastoris fed batch, glycerol, pH 3.0

Shift glycerol to methanol at pH 3

  1. The table indicates the labelling of the samples and which samples are hybridised together on one microarray for which experiment. The chip numbering is arbitrary. "Yellow" experiment indicates that identical samples were labelled with both dyes and hybridised to the same microarray to test reproducibility.