Figure 4From: Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisationNorthern blot analysis of selected genes. Exemplarily, the RNA of the pH 5.0 experiment was analysed for the expression of four genes. Actin and human trypsinogen were used as controls for an unregulated, and a methanol induced gene, respectively. MET17 (O-acetylhomoserine (thiol)-lyase) and SAH1 (S-adenosyl-L-homocysteine hydrolase) were chosen as strongly down- or upregulated genes, as determined before (table 6).Back to article page