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Figure 4 | Microbial Cell Factories

Figure 4

From: Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Figure 4

Northern blot analysis of selected genes. Exemplarily, the RNA of the pH 5.0 experiment was analysed for the expression of four genes. Actin and human trypsinogen were used as controls for an unregulated, and a methanol induced gene, respectively. MET17 (O-acetylhomoserine (thiol)-lyase) and SAH1 (S-adenosyl-L-homocysteine hydrolase) were chosen as strongly down- or upregulated genes, as determined before (table 6).

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