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Figure 2 | Microbial Cell Factories

Figure 2

From: Differential gene expression in recombinant Pichia pastoris analysed by heterologous DNA microarray hybridisation

Figure 2

Fed batch fermentations of P. pastoris. The two panels represent the two different fermentations performed: (a) The pH was kept at 5.0 throughout the fermentation. (b) The pH was let drop to 3.0 and was kept constant subsequently. (A) indicates the batch phase, the cells were growing on glycerol. The time scale starts at 25 h. (B) indicates the glycerol fed batch phase and (C) indicates the methanol fed batch phase. Methanol induces heterologous protein production and serves as a carbon source at the same time. The diamonds (â—‡) show the total yeast dry mass and refer to the left scale. The squares (â–¡) show the pH of the culture broth and refer to the right scale. The arrows indicate the time points, when the samples for microarray analysis were taken.

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