Figure 1

Statistical evaluations. (A) "yellow experiment": identical cDNA samples of P. pastoris were differently labelled and hybridised to S. cerevisiae microarrays. Normalised data of channel 532 nm (Cy3) are plotted against channel 635 nm (Cy5). The solid line represents the linear correlation. Dotted lines indicate the limits of 1.5 fold differences between two signals on one spot. More than 99% of all values vary less than 1.5 fold from each other. (B) Standard deviations of all value pairs as shown in panel A, plotted against the respective mean normalised intensities. (C) Correlation of spot intensities comparing S. cerevisiae and P. pastoris, grown in identical conditions. (D) Correlation of gene regulation. The correlation between the downregulated genes of S. cerevisiae and P. pastoris upon a shift from pH 5.0 to pH 3.5 is shown.