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Figure 8 | Microbial Cell Factories

Figure 8

From: Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

Figure 8

Cytoplasmic expression of unfused scFv After cloning in plasmid pPM210, scFv225.28S and mutants were expressed in strain TG1lon. 5 μl of soluble and insoluble extracts were analyzed by Coomassie blue staining (A) and western blot (B) using the 9E10 anti-c-myc monoclonal antibody followed by an anti-mouse alkaline phosphatase-conjugated antibody and detected with the chromogenic substrate BCIP/NBT.

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Microbial Cell Factories

ISSN: 1475-2859

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