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Figure 7 | Microbial Cell Factories

Figure 7

From: Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

Figure 7

Expression of scFv-α fusions in TG1 and TG1lon strains SDS-PAGE of soluble (5 μl : A, C & F) and insoluble extracts (5 μl ; B, D & G) of the five best clones of scFv 225.28S (A, B, C & D) and scFv HuLys11 (F & G) expressed in TG1 and TG1lon. Proteins were revealed either by Coomassie blue staining (A & B) or by western blot (C, D, F & G) using an HRP-coupled anti-polyHistidine monoclonal antibody (Sigma A7058) and detected with a commercial detection kit (Pierce Supersignal West Pico kit #14079). In panel E, the same soluble extracts than in C were tested for βgal activity.

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Microbial Cell Factories

ISSN: 1475-2859

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