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Figure 5 | Microbial Cell Factories

Figure 5

From: Directed evolution of single-chain Fv for cytoplasmic expression using the β-galactosidase complementation assay results in proteins highly susceptible to protease degradation and aggregation

Figure 5

Phenotype of the isolated mutants After four rounds of mutagenesis and selection, the five best clones of scFv 225.28S were analyzed in strain TG1. pPM175 contains the wild type scFv and pPM175R4.1 to pPM175R4.5 the five isolated mutants. T+ and T- are the same as in Fig. 2. A) Phenotype of the strains on MacConkey lactose plates; B) βgal activity measured as in Figure 1. In addition to the Miller's whole cell assay (blue), we analyzed in B the βgal activity present in soluble extracts (red).

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Microbial Cell Factories

ISSN: 1475-2859

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