Figure 11

Schematic representation of the possible in vivo fates of scFv-α protein fusion Schematic representation of folding, aggregation and degradation processes. In the scheme presented, the newly translated protein (left) is assumed to proceed through two alternative pathways: either it folds to give the folded protein (soluble native) or it evolves via a side reaction leading to a misfolded protein (misfolding). A second kinetic competition is thought to occur between degradation and aggregation of the misfolded protein. Green arrows represent the kinetic competition between folding and aggregation [44] on which the soluble reporter assays are based [18]. Red arrows represent an additional pathway leading to degradation [43] and release of free α-peptide in the cytoplasm.