Table 1 Plasmids used in this work
Plasmid | Description | Reference |
|---|---|---|
pTC191 | A derivative of pUC19 containing identical restriction sites on both sides of the polylinker | [36] |
pAM2PP | Contains the promoter-less amy gene (encoding the S. griseus α-amylase) | [24] |
pTQ217 | A derivative of pTQ207 (Günter et al. 1993) containing the desA gene of S. pilosus encoding the enzyme lysine decarboxylase | T. Schupp, personal communication |
pIJ699 | Bifunctional vector E. coli-Streptomyces of high copy number. The functional region in Streptomyces is flanked by transcriptional terminators. Allows positive selection of transformants | [25] |
pULVD10 | A derivative of pIJ699 that contains the promoter-less amy gene expressed from the saf promoter (saf p) of S. griseus | [27] |
pIJ2842 | Low-copy number promoter-probe vector for Streptomyces promoters | [26] |
pTCA | A derivative of pTC191 containing the amy gene without its promoter in a 1.8 kb DNA fragment | This work |
pTCDA | A derivative of pTCD containing the desA promoter (desA p) coupled to the 5' end of the promoter-less amy gene | This work |
pTCADi | A derivative of pTCD containing the desA promoter fused to the 3' end of the amy gene (negative control) | This work |
pUL99DA | A derivative of the multicopy pIJ699 containing the transcriptional fusion desA p-amy in the correct orientation | This work |
pUL99ADI | A derivative of pIJ699 containing the incorrect transcriptional fusion amy-desA p (non-functional) | This work |
pIJ42DA | A derivative of the low-copy pIJ2842 containing the transcriptional fusion desA p-amy in the correct orientation | This work |
pIJ42ADI | A derivative of the low-copy pIJ2842 containing the incorrect transcriptional fusion amy-desA p no functional | This work |