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Table 1 Sequences of the oligonucleotide probes used in sandwich and slot blot hybridizations. All probes are designed for S. cerevisiae.

From: Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates

Target name

Probe name

Probe sequence 5'->3'

Location of probe

 

Capture probes

  

YIL162W

SUC2-1400

UUGUAGUCUUGUGAUUUGGCAGC- (AAGAG)*

1400–1422

RDN18-2

18S rRNA-400

CUGCUGCCUUCCUUGGAUGUGGUAGCCGUU- (GAGAG)

400–429

 

Detection probes

  

YIL162W

SUC2-581

GACUUGAAUGGUUGGUUGUUGAC- (GUGAC)

581–603

RDN18-2

18S rRNA-1302

UCUCGUUCGUUAUCGCAAUUAAGCAGACAA- (GCAGC)

1302–1331

  1. * 5nt long spacer arms in brack