Figure 4
From: Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
Effect of cell lysate and total RNA on the signal of the solution hybridization. 1.5 × 1011 SUC2 in vitro transcribed RNA molecules were added to the hybridization solution containing crude cell lysate (○) or RNA extract (●) from 1 × 106 – 1 × 109 S. cerevisiae cells grown on glucose. The results are expressed as a percentage value of the signal compared to that obtained with the same amount of pure in vitro transcribed target SUC2 mRNA. Approximately 1.4 μg total RNA was extracted from 1 × 108 cells. The error bars show the ± SD of three parallel samples.