Figure 3

Comparison of extracted total RNA and crude cell lysate as sample material in the sandwich hybridization assay. 18S rRNA was used as target molecule for method evaluation. 5 ml of OD₆₀₀ = 2 yeast culture was lysed (○) or used for extraction of total RNA (●). Different dilutions of lysed yeast cells or total RNA extracted from the same number of cells were added to the sandwich hybridization solution. Fluorescence (FLU) measured with 18S rRNA probes is presented in relation to the amount yeast cells used for lysis or extraction of RNA. The error bars show the ± SD of three parallel experiments.