Figure 2From: Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysatesComparison of sandwich solution hybridization with slot blot filter hybridization assay and real-time PCR. In a) 3.1 × 107 – 7.2 × 1011 18S rRNA target molecules were added to the sandwich hybridization solution (○) or to the membrane for slot blot hybridization (●). Fluorescence (sandwich assay) and chemiluminescence (slot blot assay) signals were both generated by anti-DIG-alkaline phosphatase-FAB fragments. In b) 2 × 105 – 6 × 1010 18S rRNA target molecules were amplified by the real-time PCR reaction, using SYBR-green as detection dye (Δ). Ct is number of cycles needed to reach threshold fluorescence signal level. The error bars show the ± SD of three parallel samples.Back to article page