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Figure 2 | Microbial Cell Factories

Figure 2

From: Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates

Figure 2

Comparison of sandwich solution hybridization with slot blot filter hybridization assay and real-time PCR. In a) 3.1 × 107 – 7.2 × 1011 18S rRNA target molecules were added to the sandwich hybridization solution () or to the membrane for slot blot hybridization (). Fluorescence (sandwich assay) and chemiluminescence (slot blot assay) signals were both generated by anti-DIG-alkaline phosphatase-FAB fragments. In b) 2 × 105 – 6 × 1010 18S rRNA target molecules were amplified by the real-time PCR reaction, using SYBR-green as detection dye (Δ). Ct is number of cycles needed to reach threshold fluorescence signal level. The error bars show the ± SD of three parallel samples.

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