Figure 1
From: Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
Principle of the sandwich hybridization assay. In solution hybridization the biotin labeled capture probe and the digoxigenin labeled detection probe are first hybridized with target RNA (a) followed by hybrid immobilization on magnetic beads and detection with anti-DIG – alkaline phosphatase FAB fragments (b). Alkaline phosphatase cleaves BBTP (2'-[2-benzothiazoyl]-6'-hydroxybenzothiazole phosphate, AttoPhos®) to inorganic phosphate (Pi) and BBT (2'-[2-benzothiazoyl]-6'-hydroxybenzothiazole)