Composition of sequentially propagated original complex starter culture (panel A) and reconstituted defined starter culture (panel B). The cultures were daily propagated in sterile skimmed milk with 1% inoculum for a period 29 days (equivalent to 186 generations) at 20°C. The composition of the cultures at the end-point was measured using lineage specific qPCR primers described in . The colours indicate the genetic lineage and the percentages indicate the measured relative abundance. Data from: De Groof, Wolkers-Rooijackers and Smid, 2013, unpublished results.