Figure 2From: Expression of single-chain variable fragments fused with the Fc-region of rabbit IgG in Leishmania tarentolae Antibodies expressed and purified from T7-TR. Coomasie from the left: Molecular weight marker (SeeBlue plus2; Invitrogen), A10 scFv, LOB7 scFv, Y4A scFv, A10 scFv-rFc, and LOB7 scFv-rFc. Western blot from the left: LOB7-rFc reduced (r), and LOB7-rFc non-reduced (n-r). All antibodies seen in the Coomasie stain were separated on SDS in reducing conditions.Back to article page