Production of scopularide A in submerged culture with Scopulariopsis brevicaulis

Table 1 Biomass and maximal scopularide A production by S. brevicaulis LF580 in bioreactor cultivations

Condition	Glucose (g L^-1)	C/N (g g^-1)	Biomass (g L^-1)	Scopularide A (mg L^-1)^†	Time (h)
Static flask - YMP [8]	10	10		7	~336
O₂-limited batch-YMP^††	11	10	11	10	90
Batch – YMP	11	10	11 ± 1	29 ± 3	90
N-limited batch-YMP	21	15	12 ± 1	8	74
Batch – YMP	20	11	15 ± 2	58 ± 4	90
Batch or fed-batch-YMP	44	10	25 ± 1	142 ± 20	167
Batch or fed-batch-YMP	61	10	33 ± 1	202 ± 58	165
Batch – CSS	17	7	12	44	90
Batch – defined, glucose	17	9	12	41	87
Batch – defined, glucose	40	9	24 ± 6	44 ± 7	100
Batch – defined, xylose	40	9	25 ± 1	54 ± 2	100

S. brevicaulis LF580 was grown in YMP, CSS or defined medium (and 30 g Sea Salt L^-1) in batch or fed-batch culture at 28°C. Scopularide A was extracted from the mycelia at the times indicated. C/N values are estimated, as indicated in the methods. Average values are given, with ± sem for scopularide A and biomass, for cultivations which were carried out in replicate (n = 2 to 5). Published data from static flasks [8] is provided for comparison.
^†Determined from total dry biomass, volume of culture remaining and specific scopularide A content of the biomass (see Figure 1).
^††O₂-limited culture agitated at 300 rpm, with 0.6 vvm air.

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