Skip to main content

Table 3 Physiological parameters of the wild type P. putida KT2440 and metabolically engineered strains in batch culture grown with of glucose

From: Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida

Strain

μ max(h-1)

YX/S §(g/g)

Y2-ketoglut/S(g/g)

Ygluconate/S(g/g)

YPHA/S(g/g)

KT2440

0.56

0.22

<0.001

0.64

0.05

KT2440Δ gcd

0.42

0.23

<0.001

<0.001

0.08

KT2440- acoA

0.40

0.21

0.04

0.38

0.07

KT2440Δ gcd-acoA

0.25

0.25

<0.001

<0.001

0.11

KT2440 -pgl

0.33

0.21

0.07

0.55

0.05

KT2440Δ gcd-pgl

0.21

0.24

<0.001

<0.001

0.08

  1. § Yx/s is calculated based on the total biomass concentration (true biomass + PHA content). Standard deviations of less than 10% were obtained in 3 independent experiments.