Production of medium chain length polyhydroxyalkanoate in metabolic flux optimized Pseudomonas putida

Table 2 Monomer composition of mcl-PHA produced by metabolically engineered P. putida strains in flask experiments

Strain	C6^∞	C8^∞	C10^∞	C12^∞	C12:1^∞	C14^∞
KT2440	n.d.	11.8 ± 0.2	73.0 ± 0.1	4.7 ± 0.2	9.4 ± 0.7	0.8 ± 0.3
KT2440Δ *gcd*	n.d.	14.0 ± 0.4	71.1 ± 0.8	4.6 ± 0.9	8.5 ± 0.4	1.1 ± 0.2
KT2440- *acoA*	n.d.	13.0 ± 0.6	69.1 ± 1.2	3.4 ± 0.7	10.5 ± 0.2	3.9 ± 0.2
KT2440Δ *gcd-acoA*	n.d.	14.8 ± 0.7	70.2 ± 1.4	5.1 ± 0.3	8.8 ± 0.1	0.7 ± 0.3
KT2440-pgl	n.d.	12.2 ± 0.9	74.1 ± 1.5	3.8 ± 0.5	9.9 ± 0.8	n.d.
KT2440Δ *gcd-pgl*	n.d.	14.6 ± 0.3	73.1 ± 0.8	3.7 ± 0.2	8.1 ± 0.5	0.5 ± 0.1

n.d.: not detected, less than 0.2%.
^∞: The data were determined by GC/MS and are given as relative molar fraction (%) of C6: 3-hydroxyexanoate, C8: 3-hydroxyoctanoate, C10:3-hydroxydecanoate, C12: 3-hydroxydodecanoate, C12:1: 3-hydroxy-5-cis-dodecanoate, and C14: 3-hydroxytetradecanoate.

ISSN: 1475-2859