Figure 7From: Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate Operational stability of the immobilized cells. Batch and continuous reactions were conducted at laboratory scale in a stirred tank reactor (●) and a packed-bed column (○) using 2.04 M (70% w/v) cane sugar at 60°C and pH 5.5. The stirred tank was operated with 50 g of wet beads (8 g dry weight) in 1 L of substrate solution in successive batches repeated every 24 h. The packed-bed column was loaded with 150 g of wet beads (24 g dry weight) and fed at flow rate of 30 mL/h.Back to article page