Time course analyses of cane sugar transformation by the calcium-alginate entrapped cells. The experiment was conducted in a laboratory-scale stirred batch reactor operating at 60 (A) and 70°C (B). The beads (100 g, wet weight) were reacted with 2.04 M cane sugar in 50 mM sodium acetate buffer, pH 5.5 (1 L) in a stirred tank reactor. Samples (0.5 mL) were retrieved every hour and the sugar composition was determined by HPLC. The experiment was replicated three times. Standard deviation of the means was below 10%. Symbols represent F, fructose; G, glucose; GF, sucrose; GFF, 1-kestose.