Figure 5From: Complete sucrose hydrolysis by heat-killed recombinant Pichia pastoris cells entrapped in calcium alginate Thermal stability of periplasmic BfrA in free and entrapped cells. Samples were incubated in 50 mM sodium acetate buffer (pH 5.5) without substrate for 15 h at different temperatures. The remaining invertase activity was quantified by adding sucrose to 120 mM and reacting for 15 min at 60°C. Percentage values are relative to the activity of the free and immobilized cells kept at 4°C before the reaction. Data are the means of triplicate measurements ± standard deviation.Back to article page