Effect of biomass loading on the cell entrapment efficiency and the invertase activity. Non-viable cell suspensions at final concentrations of 50, 100, 200, 300, and 400 g/L (wet biomass) were subjected to calcium alginate entrapment. The beads invertase activity was determined for sucrose (120 mM) hydrolysis at pH 5.5 and 60°C using the DNSA method. Immobilization yield expresses the percentage of initial invertase activity which was not recovered in the entrapped cells but remained in the CaCl2 solution. Data are the means of triplicate measurements ± standard deviation. Different letters are significantly differences between invertase activity for each quantity of biomass loading (Student-Newman-Keuls test, α = 0.05).