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Table 2 Effect of substrate concentration on the bioreduction of 2-octanone to ( R )-2-octanol with Acetobacter pasteurianus GIM1.158 cells

From: Biocatalytic anti-Prelog reduction of prochiral ketones with whole cells of Acetobacter pasteurianus GIM1.158

Co-substrate

V0(×10−2 μ mol/min)

Time (h)

Yield (%)

e.e.(%)

No cosubstrate

0.4

6.0

2.0

70.2

Glucose

4.4

6.0

41.9

75.8

Glycerol

1.8

6.0

18.7

8.6

Ethanol

15.5

2.0

82.4

80.6

Isopropanol

12.2

2.0

60.5

>99.9

Methanol

n.r.

6.0

n.r.

n.r.

Fructose

n.r.

6.0

n.r.

n.r.

Sodium lactate

13.8

2.0

72.4

72.3

  1. Reaction conditions: 2 mL TEA-HCl buffer (50 mmol/L, pH 5.0), 25 mg/mL wet cells, 10 mmol/L 2-octanone, 100 mmol/L co-substrates, 35°C, 180 r/min.
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Microbial Cell Factories

ISSN: 1475-2859

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