Biocatalytic anti-Prelog reduction of prochiral ketones with whole cells of Acetobacter pasteurianus GIM1.158

Table 1 Biocatalytic asymmetric reduction of 2-octanone with various strains

Entry	Strain	V₀(×10⁻¹ μ mol/min)	Time (h)	Y^a(%)	e.e.(%)	Config.
1	Acetobacter pasteurianus GIM1.158	1.07	2.0	53.4	98.5	R
2	Acetobacter sp. CCTCC M209061	0.79	2.0	43.6	97.7	R
3	Bacillus cereus AS1.126	0.12	4.0	11.6	56.1	S
4	Pseudomonas putica GIM1.193	0.09	4.0	8.7	35.3	S
5	Candida parapsilosis CCTCCM203011	0.84	4.0	64.2	66.3	S
6	Candia tropicalis CICC1316	0.87	2.0	91.5	10.5	S
7	Saccharomyces cerevisiae GIM 2.34	0.45	2.0	45.4	>99.9	S
8	Rhodotorula sp. AS2.2241	0.55	2.0	54.7	22.3	S
9	Pseudomonas oleovorans GIM1.304	0.60	2.0	60.3	18.1	S

Reaction conditions: 2 mLTEA-HCl (50 mmol/L, pH 5.0), 25 mg/mL wet free cells, 10 mmol/L 2-octanone, 100 mmol/L isopropaanol, 30°C, 180 r/min.
Y^a: The maximum yield.

ISSN: 1475-2859