Figure 1
Effect of the C/N ratio on growth and lipid production of C. freyschussii ATCC 18737. C. freyschussii ATCC 18737 was cultured in GMY medium containing 3 g/L yeast extract and different glycerol concentration, in order to obtained different C/N ratios. Biomass was harvested and lipid content was analyzed at the latest after 72 h, unless glycerol was depleted earlier. Residual glycerol, if any, is reported in brackets. A, final cell count (cyan); B, final concentrations: biomass (purple) and lipids (grey); C, yield coefficients: lipid/biomass (YL/X, green) and lipid/glycerol (YL/S, yellow). Values are means ± SD, n = 3. Within each series, means are significantly different (P < 0.05), unless they have a common superscript.