Figure 4

Schematic location of peptides identified from proteinase sequences after cell surface shaving. Cell surface proteins of S. thermophilus LMD-9, PrtH⁺, PrtH⁺WANS strains were hydrolysed by trypsin and peptides were identified by chromatography coupled on line with ESI quadrupole-orbitrap tandem mass spectrometry. A. Peptides corresponding to CEPs were aligned with PrtS, PrtH⁺ and PrtH⁺WANS protein sequences. Peptide sequences are represented with blue bars under the corresponding domains of CEPs: S, PP, PR, A, B, W and AN. B. Peptides identified (blue bars) around the cleavage site between PP and PR domains of S. thermophilus LMD-9 PrtS CEP. Amino acids and their position from the S domain of PrtS are indicated.