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Table 2 Hydroxylation activity of B. megaterium ATCC13368 monitored over a period of 48 hours

From: Steroid conversion with CYP106A2 – production of pharmaceutically interesting DHEA metabolites

16 h

24 h

36 h

48 h

100%

105 ± 3%

136 ± 42%

84 ± 22%

  1. Cells were cultivated in 50 ml Erlenmeyer flasks at 30°C and 150 rpm. 500 μl samples were taken at time points indicated. Conversions were performed with 100 μM substrate in 1.5 ml reaction tubes at 30°C for 60 min. The reactions were quenched and extracted with ethyl acetate and the steroid conversion was analyzed with HPLC. The activity of B. megaterium after 16 h cultivation was defined as 100%. Results are shown as mean values and deviations are from two independent experiments.
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Microbial Cell Factories

ISSN: 1475-2859

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