Figure 6From: Steroid conversion with CYP106A2 – production of pharmaceutically interesting DHEA metabolites HPLC chromatograms of DHEA conversion using resting cells B. megaterium ATCC13368 (wt) and MS941_pSMF2.1CAA (CAA). Cultures were incubated at 30°C and 150 rpm for 24 h, washed once and suspended in 50 ml potassium phosphate buffer adjusted to pH 7.4. Heterologous protein expression in B. megaterium MS941_pSMF2.1CAA cells was induced with 0.5% (w/v) xylose. DHEA (400 mM) was added as ethanolic solution (5% v/v). Samples were taken directly after substrate addition (CAA 0 h, wt 0 h) and after 1 h (CAA 1 h, wt 1 h). No DHEA (RT = 11.5 min) was detected in the 1 h samples.Back to article page