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Figure 2 | Microbial Cell Factories

Figure 2

From: Steroid conversion with CYP106A2 – production of pharmaceutically interesting DHEA metabolites

Figure 2

Difference spectroscopy measurements of DHEA and androstenedione binding to CYP106A2. a) Difference spectra of DHEA with CYP106A2 in tandem cuvettes recorded from 350 to 500 nm. 0, 75, and 100 μM DHEA solved in DMSO were used. b) Difference spectra of androstenedione recorded from 350 to 500 nm in tandem cuvettes. Androstenedione was titrated from 0 to 600 μM into a 10 μM CYP106A2 solution. c) The binding constant KD was calculated by plotting the peak-to-through difference and subsequent hyperbolic fit (solid line). The apparent dissociation constant of androstenedione was calculated to be KD 92 ± 11 μM. The results represent mean values and standard deviations from three independent titrations.

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