Table 1 List of plasmids

pGEM®-T

Ap^r

Promega, Madison, WI

pUC18-OVA

1.2 kb ovalbumin gene in pUC18

Ap^r

[25]

pZS1201

pGEM®-T Easy Vector - OVA-CD4

Ap^r

This study

pnirBLTBsp-MisL

pnirB for LTsp-MisL, colE1-like ori

Ap^r

[7]

pZS1202

pnirBLTBsp-MisL-OVA-CD4

Ap^r

This study

pZS1203

pGEM®-T Easy Vector -spiC p

Ap^r

This study

pZS1204

pZS1202 with spiC p

Ap^r

This study

pCS238

pACYC184 with ccdAB-flmAB

Cm^r

[10]

pZS1205

pZS1204 with ccdAB-flmAB

Ap^r

This study

pZS1205-2

pZS1205 with [OVA-CD4]x2

Ap^r

This study

pZS1205-4

pZS1205 with [OVA-CD4]x4

Ap^r

This study