Highly efficient L-lactate production using engineered Escherichia coli with dissimilar temperature optima for L-lactate formation and cell growth

Table 2 Primers used in this study

Primers	Sequence (5'-3') restriction sites are italic/underlined	Restriction sites
LdhA1	TAAGAATTC ^aTTATGAAACTCGCCGTTTATAGCACA	EcoR I^a
LdhA2	CTTGAATTC ^a AAGCTT ^aGCTGCCGGAAATCATCATTTTTT	EcoR I^a, Hin dIII^a
LdhA3	GGGCAGCCCGAGCGTCATCAG
LdhA4	GCTGCCGGAAATCATCATTTTTT
BcoaLDH1	CCGGATCC ^aAATCAGGGTGTTGCAGAAGAGCTTG	Bam HI^a
BcoaLDH4	GCGGAATTC ^aTTACAATACAGGTGCCATCGTTTCT	EcoR I^a
LcaLDH1	CGCGGATCC ^aAGTATTACGGATAAGGATCAC	Bam HI^a
LcaLDH4	CGCCTGCAG ^aTCCTGTTCTTCGTTTG	PstI ^a
StrbLDH1	CGCGGATCC ^aACTAAACAACACAAAAAAG	Bam HI^a
StrbLDH2	CCGGAATTC ^aTACAGGGATTGTTGCCGCA	EcoR I^a
LldD1	GGCCCGGG ^aCATGATTATTTCCGCAGCCA	Sma I^a
LldD2	GGCCCGGG ^aCAGGCAACTCTTTACCCAGCCC	Sma I^a

ISSN: 1475-2859