Highly efficient L-lactate production using engineered Escherichia coli with dissimilar temperature optima for L-lactate formation and cell growth

Table 1 Strains and plasmids used in this study

Strains	Relevant characteristics	Source or reference
Escherichia coli B0013-070	B0013, Δack-pta, Δpps, ΔpflB, Δdld, ΔpoxB, ΔadhE, ΔfrdA	[10]
E. coli B0013-080C	B0013-070, ΔldhA	This study
E. coli B0013-090B1	B0013-080C, ΔlldD:: ldhA p-ldh _Lca	This study
E. coli B0013-090B2	B0013-080C, ΔlldD:: ldhA p-ldh _Strb	This study
E. coli B0013-090B3	B0013-080C, ΔlldD:: ldhA p-ldh _Bcoa	This study
Bacillus coagulans B1821	Wild type	CICIM-CU^a
Lactobacillus casei B1192	Wild type	CICIM-CU^a
Steptococcus. bovis 1.1624	Wild type	CGMCC^b
Plasmids	Relevant characteristics	Source or reference
pSK-dif Gm^R	dif Gm, bla	[20]
pMD18-T simple	bla; TA cloning vector	TaKaRa, Japan
pLDHex	bla; P_ldhA, T_ldhA	This study

^aThe Culture and Information Center of Industrial Microorganisms of China Universities, Jiangnan University, China.
^bChina General Microbiological Culture Collection Center, Institute of Microbiology, CAS, Beijing, China.

ISSN: 1475-2859