The growth characteristics of E . coli strains 090B1 (a), 090B2 (b) and 090B3 (c) at various temperatures. For the experiments, a shaking flask fermentation test was carried out in a 250-ml flask with working volume of 50 ml at various temperatures from 25°C, 30°C, 34°C, 37°C, 40°C, 42°C, 45°C to 50°C for up to 14 h. Sampling was carried out in every 2 hours. The cell density was colorimetrically analyzed at 600 nm. The experiments were carried out in triplicates.