A bacteriocin gene cluster able to enhance plasmid maintenance in Lactococcus lactis

Table 1 Growth and bacteriocin production of L. lactis NZ9000 transformed with Lcn972-expressing plasmids

Plasmid	μ (h⁻¹)	g (h)	ODmax	Lcn972 activity (AU^a/mL)	MIC^bLcn972 (AU/mL)
pBL1 (WT)	1.04 ± 0.13	0.67 ± 0.08	3.52 ± 0.21	6400	1600
pLDH	1.02 ± 0.12	0.69 ± 0.08	3.44 ± 0.37	800	1600
pHYB	0.67 ± 0.06	1.04 ± 0.09	3.18 ± 0.47	200	1600
pOrf4	1.01 ± 0.10	0.69 ± 0.07	3.25 ± 0.36	400	1600
pDorf4	0.88 ± 0.04	0.79 ± 0.03	3.45 ± 0.30	400	800

^aAU: Arbitrary Units.
^bMIC: Minimal Inhibitory Concentration. The MIC of Lcn972 for L. lactis NZ9000 is 12.5 AU/mL.
Growth rate (μ), generation time (g), maximal OD₆₀₀ (ODmax) and production and immunity to Lcn972 are shown. Results are the average ± standard deviation from, at least, two independent growth experiments in GM17.

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