Figure 4
From: A bacteriocin gene cluster able to enhance plasmid maintenance in Lactococcus lactis
Integration of the plasmid pOrf4 in the chromosome of L. lactis NZ9000. (A) Results of the PCR strategy to define the region of pOrf4 involved in the molecular reorganization with the chromosome. Primer pairs orf4-F/lcn2, ery5/orf4-R, eryrepF-F/repE-R, and repE-F/repE2-R were used in PCRs A, B, C and D, respectively. (B) Presence of the insertion sequences (black arrows) IS981 and IS1216 as revealed by direct sequencing of the chromosome of L. lactis 200 g and L. lactis 400 g, respectively.