Figure 2

Segregational stability of Lcn972-expressing plasmids in L. lactis NZ9000 grown without erythromycin (Em). All the experiments were started from fresh overnight cultures in GM17Em (except pBL1). Experiments were carried out once except for pIL252 (in A), that was performed three times (only one representative curve is shown), and for pOrf4 (in B), where two independent Em-resistant colonies (−a, −b) were tested. L. lactis 200 g-a is a Em-resistant Lcn972-producing colony isolated from L. lactis pOrf4 after 200 generations.