Skip to main content
Figure 3 | Microbial Cell Factories

Figure 3

From: Flux analysis of the Lactobacillus reuteri propanediol-utilization pathway for production of 3-hydroxypropionaldehyde, 3-hydroxypropionic acid and 1,3-propanediol from glycerol

Figure 3

Multi-step variable-volume fed-batch biotransformation of glycerol using resting cells of L. reuteri. Multi-step variable-volume fed-batch biotransformation of glycerol using resting cells of wild-type (A & C) and RPRB3007 (B & D) strains of L. reuteri (6 gCDW/L). Experiments were conducted at pH 5 (A & B) and pH 7 (C & D). After 1 h of batch biotransformation, glycerol (50 g/L) was fed at a rate of 0.6 ggly/h, 1.0 ggly/h and 1.9 ggly/h, respectively, for determination of the maximum flux through the reductive and oxidative branches of the Pdu pathway at pH 5. The corresponding feeding rates were 0.6 ggly/h, 1.6 ggly/h and 2.5 ggly/h, respectively, at pH 7. Consumed glycerol (), produced 3HP (■), 3HPA () and 1,3PDO (▲) are presented.

Back to article page