Table 2 BioBricks and primers used to obtain a genetic construct for starch hydrolysis
Name | Description or Sequence (5'-3') | Reference |
|---|---|---|
BBa_B0030 | Strong RBS | [31] |
BBa_B1002 | Artificial double-transcriptional terminator | [32] |
fhuDsp | Signal peptide from fhuD, obtained by PCR with the primers spF and spR | This work |
malEp | Promoter from malE, obtained by PCR with the primers prF and prR | This work |
amyA | amyA gene, obtained by PCR with the primers amF and amR | This work |
spF | GTTTCTTCGAATTC GCGGCCGCTTCTAGA TGAGCGGCTTACCTCTTAT | This work |
spR | GTTTCTTCCTGCAG CGGCCGCTACTAGTCGCGTGGGCGGTATTCATCT | This work |
prF | GTTTCTTCCTGCAG CGGCCGCTACTAGTAGCGTGCACCTGTTTTTATTTTCCTAATCTATGGTCC | This work |
prR | GTTTCTTCGAATTC GCGGCCGCTTCTAGA GTGGCTTAAATCCTCCACCCC | This work |
amF | GTTTCTTCGAATTC GCGGCCGCTTCTAGA CGTAATCCCACGCTGTTACA | This work |
amR | GTTTCTTCCTGCAG CGGCCGCTACTAGTATTATTAAATCACCTCTTCGATAACCC | This work |