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Table 1 Strains and plasmids

From: Engineering Escherichia coli K12 MG1655 to use starch

Strain/plasmid

Genotype or description

Reference

Bm

Bacillus megaterium

[24]

WT

Escherichia coli K-12 MG1655

[25]

WTa1-6

WT, starch-adapted strains

This work

AmyC

WT with the backbone plasmid pSB1A2

This work

Amy0

WT with pAM

This work

Amy6

WTa6 with pAM

This work

Amy(−)

WT with amyA deleted

[26]

pSB1A2

BioBricks backbone plasmid

[27]

pSB1AK3

BioBricks backbone plasmid

[28]

pAM

pSB1A2 with engineered amyA

This work