Table 7 Bacterial strains and vectors used in this study
Strain or vector | Description | Source or reference |
|---|---|---|
Strains | ||
E. coli MegaX DH10B T1R | F- mcr A Δ(mrr-hsd RMS-mcr BC), Φ80lac ZΔM15 Δlac X74 rec A1 end A1 ara D139 Δ(ara, leu)7697 gal U gal K λ− rps L nup G ton A | Life Technologies |
E. coli Tuner™ | F– ompT hsdSB (rB– mB–) gal dcm lacY1 | Novagen |
IKA3C6 | MegaX DH10B T1R E. coli carrying metagenomic library clone with α-amylase activity | This study |
IKA16D10 | MegaX DH10B T1R E. coli carrying metagenomic library clone with α-amylase activity | This study |
IKA28E6 | MegaX DH10B T1R E. coli carrying metagenomic library clone with α-amylase activity | This study |
IKA3H5 | MegaX DH10B T1R E. coli carrying metagenomic library clone with β-galactosidase activity | This study |
IKA17E2 | MegaX DH10B T1R E. coli carrying metagenomic library clone with β-galactosidase activity | This study |
Plasmids | ||
pGNS-BAC | Gram negative BAC shuttle vector | [59] |
mod.pGNS-BAC | pGNS-BAC modified to include a multiple cloning site with four unique restriction sites (Apa LI, Bsp DI, Bbv CI and Nsi I) into the Hind III site | This study |
pET21b | E. coli expression vector | Novagen |