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Table 6 Comparison of β-galactosidases

From: Discovery of novel enzymes with industrial potential from a cold and alkaline environment by a combination of functional metagenomics and culturing

 

BGalI17E2

BGalI3H5

A. ikkensis β-galactosidase

E. coli LacZ

Monomeric size

455aa

820aa

1,041aa

1,029aa

52.75 kDa

92.25 kDa

119.13 kDa

116.97 kDa

GH family

1

2

2

2

Closest relatives

Clostridium hathewayi (53% identity) [GenBank:WP_006771328]

Roseburia hominis (51% identity) [GenBank:YP_004838333]

-

-

Arg

3.3%

4.5%

4.0%

6.4%

Arg/Arg + Lys ratio

0.31

0.44

0.42

0.77

Pro

3.7%

3.3%

4.8%

6.1%

Active site

WFAE YTKVM

LVTE YNGHM

ILCE FSHAM

ILCE YAHAM

  1. Comparison of the β-galactosidases from the two library clones, IKA17E2 (BGalI17E2) and IKA3H5 (BGalI3H5), to β-galactosidases from the psychrotrophic A. ikkensis[45] and the mesophilic E. coli (LacZ) [48]. Active site glutamic acid (E) highlighted in bold.