Table 5 Primers used to create and verify the knock-out strain C. werkmanii DSM17579 ∆ dhaD
From: 1,3-propanediol production with Citrobacter werkmanii DSM17579: effect of a dhaD knock-out
Primer name | Sequence (5’ → 3’) |
|---|---|
Fw-dhaD-in-P1 | AGAGAAGGTACTCAATGGTCTGCATAGCCATGACATTAGCTGCCACGCGGGTGTAGGCTGGAGCTGCTTC |
Rv-dhaD-in-P2 | AGCCACGGCCATGATCTTGCCATCAATGTCATCTTTTACGCCCATTTCCGCCATATGAATATCCTCCTTAG |
Fw-dhaD-in-out | GGTCTGCATAGCCATGACATTAGC |
Rv-dhaD-in-out | TAGCCACGGCCATGATCTTG |
SPrimer 46TA | TTCCGTGTCGCCCTTATTCC |
SPrimer46 TB | TAGTGTATGCGGCGACCGAG |