Figure 4

Consumed co-substrate concentration (blank), residual glycerol concentration (slanted lines) and final PDO concentration (crossed lines). C. werkmanii DSM17579 ∆dhaD cultures were grown on flask scale under anaerobic conditions in minimal medium with glycerol and different co-substrates (0.33 molar ratio co-substrate/glycerol). The solid line represents the initial glycerol concentration (0.163 M), the dashed line the initial co-substrate concentration (0.065 M). The errors represent the standard deviation calculated from 2 independent experiments.