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Table 1 Codon distribution of wt and synthetic taqIIRM sequences

From: Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase

aa

Codon

Fraction in E. coli 1

WT

SYN

aa

Codon

Fraction in E. coli 1

WT

SYN

Ala (A)

GCU

0.35

5

93

Leu (L)

CUG

0.83

52

145

GCA

0.28

2

0

CUC

0.07

63

0

GCG

0.28

24

0

CUU

0.04

11

0

GCC

0.10

62

0

UUG

0.03

9

0

UUA

0.02

2

0

CUA

0.00

8

0

Arg (R)

CGU

0.74

7

108

Lys (K)

AAA

0.74

11

39

 

CGC

0.25

29

0

AAG

0.26

28

0

CGA

0.01

1

0

    

AGG

0.00

31

0

    

AGA

0.00

3

0

    

CGG

0.00

37

0

    

Asn (N)

AAC

0.94

17

19

Met (M)

ATG

1

9

9

AAU

0.06

2

      

Asp (D)

GAC

0.67

41

49

Phe (F)

UUC

0.76

35

43

GAU

0.33

8

0

UUU

0.24

8

0

Cys (C)

UGC

0.51

1

2

Pro (P)

CCG

0.77

14

94

UGU

0.49

1

0

CCA

0.15

4

0

CCU

0.08

14

0

CCC

0.00

62

0

Gln (Q)

CAG

0.86

27

33

Ser (S)

UCC

0.37

14

0

CAA

0.14

6

0

UCU

0.34

0

36

AGC

0.20

12

0

UCG

0.04

4

0

AGU

0.03

6

0

UCA

0.02

0

0

Glu (E)

GAA

0.78

33

122

Thr (T)

ACC

0.55

24

37

GAG

0.22

89

0

ACU

0.35

4

0

ACG

0.07

7

0

ACA

0.04

2

0

Gly (G)

GGU

0.59

4

91

Trp (W)

UGG

1

23

23

GGC

0.39

33

0

GGG

0.02

37

0

GGA

0.00

17

0

His (H)

CAC

0.83

16

17

Tyr (Y)

UAC

0.75

43

43

CAT

0.17

1

0

 

UAU

0.25

0

0

Ile (I)

AUC

0.83

20

24

Val (V)

GUU

0.51

5

78

AUU

0.17

0

0

GUA

0.26

8

0

AUA

0.00

4

0

GUG

0.16

31

0

      

GUC

0.07

34

0

  1. 1Fraction of relative occurrences of the codon in its synonymous codon family [33]. Bold underlined – codons selected for syn-TaqII construction. Bold italics underlined – Ser codon, which is the most frequently used in E. coli.