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Figure 5 | Microbial Cell Factories

Figure 5

From: Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase

Figure 5

Isolation of recombinant syn-TaqII protein from E. coli . Lane M, protein marker (Thermo Fisher Scientific/Fermentas); lane 1, crude lysate; lane 2, supernatant after incubation at 65°C; lane 3, supernatant after PEI; lane 4, 30-50% ammonium sulphate (AmS) fractionation; lane 5, P11 chromatography; lane 6, DEAE chromatography; lane 7, size exclusion chromatography; lane 8, recombinant wt-TaqII protein.

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Microbial Cell Factories

ISSN: 1475-2859

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