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Figure 2 | Microbial Cell Factories

Figure 2

From: Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase

Figure 2

Secondary structure of the first 200 nt of taqIIRM mRNA generated by Mfold Web Server [31, 32]. (A) Structure of initial taqIIRM mRNA fragment before codon optimization (revised free energy: dG = −84.5 kcal/mol). (B) Structure of initial taqIIRM mRNA fragment after codon optimization (revised free energy: dG = −63.33 kcal/mol).

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