Skip to main content
Figure 1 | Microbial Cell Factories

Figure 1

From: Modified ‘one amino acid-one codon’ engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase

Figure 1

Differences in DNA sequences of the synthetic and wt recombinant taqIIRM genes. The predicted aa sequence of the 125.7 kDa TaqII protein is indicated in capital letters. The DNA sequence of the wt-taqIIRM gene is indicated in blue italics. The DNA sequence of the syn-taqIIRM gene is shown in black bold letters and the changed bases are marked in red. The crucial amino acids of the catalytic centres are dark red, bold and underlined. The functional protein domains are marked as follows: REase domain in blue, helical domain in light green, MTase domain in dark green and the potential TRD region in brown. Numbering of nt of taqIIRM gene variants and polypeptide aa starts as ‘1’ with the beginning (ATG) of taqIIRM ORF.

Back to article page