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Table 4 The E. coli strains used in this study

From: Increased 3-hydroxypropionic acid production from glycerol, by modification of central metabolism in Escherichia coli

Strain

Genotype

Source

BW25113

F−, λ−, lacI q rrnB T14 ΔlacZ WJ16 hsdR514 ΔaraBA-D AH33 ΔrhaBAD LD78

Datsenko and Wanner [43]

JW3890

The same as BW25113 but ΔtpiA::kan

Baba et al.[44]

BW25113 Δ zwf::cat

The same as BW25113 but Δzwf::cat

Nakahigashi et al.[45]

BW25113 Δ yqhD::tet

The same as BW25113 but ΔyqhD::tet

This study

MG1655(DE3)

F−, λ−, rph-1, λ (DE3[lacI lacUV 5-T 7 gene l indl sam 7 nin5])

This study

TK52

MG1655(DE3) transformed with pTrc99A/aldH and pCDFDuet-1/dhaB-gdrAB

This study

TK52t

The same as TK52 but Δtpi::kan

This study

TK52z

The same as TK52 but Δzwf::cat

This study

TK52tz

The same as TK52 but ΔtpiA::kan Δzwf::cat

This study

TK52tzy

The same as TK52 but ΔtpiA::kan Δzwf::cat ΔyqhD::tet

This study