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Table 2 Bioreductions of alkyl-substituted cyclohex-2-enones (substrates 4a, 5a, 6a, and 7a # ) with recombinant S. cerevisiae BY4741 ΔOye2 bearing heterologous OYE genes

From: Recombinant S. cerevisiae expressing Old Yellow Enzymes from non-conventional yeasts: an easy system for selective reduction of activated alkenes

OYE gene source 4a → 4b 5a → 5b 6a → 6b
  Time Yield Time Yield Time Yield (S)-6b
  h % h % h % e.e.%
C. castellii DBVPG3704 2 ≥ 99 2 76 72 < 5 n.d.
C. sake DBVPG6162 1 ≥ 99 1 95 48 75 ≥ 98
K. exigua L10 2 ≥ 99 2 92 72 23 ≥ 98
K. lodderae DBVPG6308 2 ≥ 99 2 84 72 25 ≥ 98
K. spencerorum DBVPG6748 2 ≥ 99 1 95 48 60 ≥ 98
Nak. bacillisporus DBVPG6945 1 ≥ 99 2 94 72 24 ≥ 98
Nau. castellii DBVPG6298 2 ≥ 99 2 95 48 53 ≥ 98
S. cerevisiae BY4741 (Oye2) 1 ≥ 99 2 96 48 45 ≥ 98
S. cerevisiae L12 2 ≥ 99 1 97 48 40 ≥ 98
  1. Data are reported as molar conversion (%). #No conversion of compound 7a was observed in the first 72 h. 15-20% of compound 5d was also observed; product 6c was always less than 15%. The experiment were performed in triplicate, the yield standard deviations (SD) were always less than 4%, and the ee% SD were always less than 3%.